Journal: Scientific Reports

Article Title: Whole-transcriptome analysis of atrophic ovaries in broody chickens reveals regulatory pathways associated with proliferation and apoptosis

doi: 10.1038/s41598-018-25103-6

Figure Lengend Snippet: The Hierarchical Cluster Analysis of differentially expressed protein-coding transcripts ( A ), miRNAs ( B ), and lncRNA transcripts ( C ).

Article Snippet: Briefly, after miRNA were separated from ovaries using the mirVana miRNA isolation kit (Abcam Inc., Cambridge, UK), 3 μg of miRNA were subjected to reverse transcription with the One Step PrimeScript® miRNA cDNA Synthesis Kit (Tiangen, China).

Techniques:

Journal: Scientific Reports

Article Title: Whole-transcriptome analysis of atrophic ovaries in broody chickens reveals regulatory pathways associated with proliferation and apoptosis

doi: 10.1038/s41598-018-25103-6

Figure Lengend Snippet: Overview of small RNA sequencing in the chicken ovary. ( A ) Portions of small RNA types in the clean reads. The percent of miRNA is approximately 73%, and the other 28% included rRNA, scRNA, snRNA, snoRNA, tRNA, simple repeats, exon sense/antisense and intron sense/antisense. ( B ) Relative frequency of different types of small RNAs in the atrophic ovary (AO) and normal ovary (NO). ( C ) Size distribution of all miRNAs. The X-axis depicts their length (nt), and the Y-axis represents frequency (%). ( D ) The relative proportion of the top 10 miRNAs in the total amount of miRNA.

Article Snippet: Briefly, after miRNA were separated from ovaries using the mirVana miRNA isolation kit (Abcam Inc., Cambridge, UK), 3 μg of miRNA were subjected to reverse transcription with the One Step PrimeScript® miRNA cDNA Synthesis Kit (Tiangen, China).

Techniques: RNA Sequencing

Journal: Scientific Reports

Article Title: Whole-transcriptome analysis of atrophic ovaries in broody chickens reveals regulatory pathways associated with proliferation and apoptosis

doi: 10.1038/s41598-018-25103-6

Figure Lengend Snippet: Sixteen intersection genes and their corresponding pathways and differentially expressed miRNAs * .

Article Snippet: Briefly, after miRNA were separated from ovaries using the mirVana miRNA isolation kit (Abcam Inc., Cambridge, UK), 3 μg of miRNA were subjected to reverse transcription with the One Step PrimeScript® miRNA cDNA Synthesis Kit (Tiangen, China).

Techniques:

Journal: Scientific Reports

Article Title: Whole-transcriptome analysis of atrophic ovaries in broody chickens reveals regulatory pathways associated with proliferation and apoptosis

doi: 10.1038/s41598-018-25103-6

Figure Lengend Snippet: The miRNA-gene-pathway network between sixteen intersection genes, and their corresponding pathways and differentially expressed miRNAs. Hexagon, round rectangle and ellipse indicate pathway, gene and miRNA, respectively. Red and green mean up- and down-regulation, respectively.

Article Snippet: Briefly, after miRNA were separated from ovaries using the mirVana miRNA isolation kit (Abcam Inc., Cambridge, UK), 3 μg of miRNA were subjected to reverse transcription with the One Step PrimeScript® miRNA cDNA Synthesis Kit (Tiangen, China).

Techniques:

Journal: Scientific Reports

Article Title: Whole-transcriptome analysis of atrophic ovaries in broody chickens reveals regulatory pathways associated with proliferation and apoptosis

doi: 10.1038/s41598-018-25103-6

Figure Lengend Snippet: An overview of the competing endogenous RNA (ceRNA) network. Rectangle, ellipse and V indicate miRNA, protein-coding transcript and lncRNA transcript, respectively. Green and red indicate down- and up-regulation, respectively.

Article Snippet: Briefly, after miRNA were separated from ovaries using the mirVana miRNA isolation kit (Abcam Inc., Cambridge, UK), 3 μg of miRNA were subjected to reverse transcription with the One Step PrimeScript® miRNA cDNA Synthesis Kit (Tiangen, China).

Techniques: